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Human monocyte interaction with biomedical polymers: Induction of monocyte-derived growth factors
- Publication Year :
- 1991
-
Abstract
- Cellular interactions which occur at tissue implant interfaces may determine the biocompatibility of implanted devices. These studies investigate the macrophage and its products, which are hypothesized to be partially responsible for the long term biocompatibility of implanted materials. Alterations in the production of cytokines and growth factors IL-1-B, IL-1-A, TNF-A, TGF-A, TGF-B and IL-6 by activated monocytes in response to biomedical polymers were evaluated. The polymers were studied with or without preadsorbed human blood proteins IgG, fibrinogen, fibronectin, hemoglobin and albumin due to the initial protein adsorption event upon implantation of foreign materials in vivo. The biomedical polymers Biomer, Polyethylene, Polydimethylsiloxane, expanded Polytetrafluoroethylene and Dacron were selected due to their current medical application. These studies utilized two different controls to evaluate the data: nonprotein preadsorbed polymers and tissue culture treated polystyrene due to the nature of the in vitro assay system. The results of these studies show that there was a polymer and protein dependent alteration in the activity of activated monocytes/macrophages at the surface of bio medical polymers determined by alterations in monocyte secretion of IL-1-B, TGF-A and IL-6. The production of TNF-A, TGFB and IL-1-A was similar for all polymer and protein combinations. Down regulation of functional IL-1 activity was observed when protein preadsorbed polymers were compared to the protein preadsorbed polystyrene. Enhancement in functional IL-1 activity was observed when the polymers were compared to their respective nonprotein preadsorbed polymer controls. The monocyte culture supernatants stimulated fibroblast proliferation in a protein and polymer dependent manner. This suggests that certain polymers and adsorbed proteins may result in decreased fibroblast proliferation which may be important when considering fibroplasia, wound healing and tissue encapsulation of implanted materials. These studies showed that activated monocytes/macrophages are altered by protein preadsorbed biomedical polymers. Alterations in monocyte activity appeared to be dependent on the polymer, adsorbed protein and cytokine evaluated. These studies support the proposed multidimensional events which occur at tissue implant interfaces in vivo
- Subjects :
- Health Sciences, Pathology
Biomedical polymers
Monocyte-derived growth factors
Subjects
Details
- Language :
- English
- Database :
- OpenDissertations
- Publication Type :
- Dissertation/ Thesis
- Accession number :
- ddu.oai.etd.ohiolink.edu.case1059154908