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A bioassay for NSILA-S in individual serum samples and its relationship to somatotropin.
- Source :
-
The Journal of clinical endocrinology and metabolism [J Clin Endocrinol Metab] 1976 Nov; Vol. 43 (5), pp. 1164-9. - Publication Year :
- 1976
-
Abstract
- A practical bioassay for the acid-ethanol soluble non-suppressible insulin-like activity (NSILA-S) of individual serum samples has been developed utilizing the incorporation of 14C-glucose into the lipid faction of isolated adipocytes. NSILA-S activity was correlated with somatotropin status. Thus, the mean potencies (+/-SD) relative to an extract of pooled normal human serum were: normal samples 1.11 +/- 0.14, acromegalic 2.91 +/- 0.72, and somatotropin deficient 0.13 +/- 0.06. This variation in NSILA-S was not due to variability in extraction recoveries. The within assay precision was 9% (coefficient of variation) and the between assay 23%. This method allows the simultaneous extraction and processing of relatively large numbers of samples, and compares favorably with other more complex methods. Because of the evidence that NSILA-S may be related to the somatomedins, the present method should provide a simpler and more reliable alternative to the cartilage bioassays used to measure somatomedin activity.
Details
- Language :
- English
- ISSN :
- 0021-972X
- Volume :
- 43
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of clinical endocrinology and metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 993319
- Full Text :
- https://doi.org/10.1210/jcem-43-5-1164