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Epitope tagging.

Authors :
Jarvik JW
Telmer CA
Source :
Annual review of genetics [Annu Rev Genet] 1998; Vol. 32, pp. 601-18.
Publication Year :
1998

Abstract

Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. This review discusses the major advantages and limitations of epitope tagging and describes a number of recent applications. Major areas of application include monitoring protein expression, localizing proteins at the cellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Recently the method has also found use in transgenic and gene therapy studies and in the emerging fields of functional genomics and proteomics.

Details

Language :
English
ISSN :
0066-4197
Volume :
32
Database :
MEDLINE
Journal :
Annual review of genetics
Publication Type :
Academic Journal
Accession number :
9928493
Full Text :
https://doi.org/10.1146/annurev.genet.32.1.601