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Sequence and expression in Escherichia coli of the coat protein gene of the dwarfing strain of soybean dwarf luteovirus.
- Source :
-
Virus genes [Virus Genes] 1998; Vol. 17 (3), pp. 207-11. - Publication Year :
- 1998
-
Abstract
- The nucleotide sequence of the coat protein gene of the dwarfing (D) strain of soybean dwarf luteovirus (SbDV) was determined from cloned cDNA. The gene contains 600 nucleotides and encodes a protein of 200 amino acids with a calculated molecular mass of 22.2 kDa. A major portion of the coat protein open reading frame (ORF) was expressed in Escherichia coli as a pET fusion protein and the product was detected by western blot analysis using SbDV-D polyclonal antibodies. Comparison of the deduced coat protein amino acid sequence to that from the yellowing (Y) strain of SbDV demonstrated 88% identity.
Details
- Language :
- English
- ISSN :
- 0920-8569
- Volume :
- 17
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Virus genes
- Publication Type :
- Academic Journal
- Accession number :
- 9926396
- Full Text :
- https://doi.org/10.1023/a:1008012219123