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Protein-DNA complexes in mycobacteriophage L5 integrative recombination.
- Source :
-
Journal of bacteriology [J Bacteriol] 1999 Jan; Vol. 181 (2), pp. 454-61. - Publication Year :
- 1999
-
Abstract
- The temperate mycobacteriophage L5 integrates site specifically into the genomes of Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium bovis bacillus Calmette-Guérin. This integrative recombination event occurs between the phage L5 attP site and the mycobacterial attB site and requires the phage-encoded integrase and mycobacterial-encoded integration host factor mIHF. Here we show that attP, Int-L5, and mIHF assemble into a recombinationally active complex, the intasome, which is capable of attB capture and formation of products. The arm-type integrase binding sites within attP play specialized roles in the formation of specific protein-DNA architectures; the intasome is constructed by the formation of intramolecular integrase bridges between one pair of sites, P4-P5, and the attP core, while an additional pair of sites, P1-P2, is required for interaction with attB.
- Subjects :
- Base Sequence
Binding Sites
Chromosomes, Bacterial genetics
DNA, Bacterial genetics
DNA, Bacterial metabolism
Integrases metabolism
Lysogeny
Mycobacterium bovis genetics
Mycobacterium smegmatis genetics
Mycobacterium tuberculosis genetics
Recombination, Genetic
Viral Proteins genetics
Viral Proteins metabolism
Mycobacteriophages genetics
Mycobacteriophages metabolism
Mycobacterium bovis virology
Mycobacterium smegmatis virology
Mycobacterium tuberculosis virology
Virus Integration
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 181
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 9882658
- Full Text :
- https://doi.org/10.1128/JB.181.2.454-461.1999