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Quantitative anhydrous mercaptolysis of algal galactans followed by HPLC of component sugars.
- Source :
-
Journal of biochemistry [J Biochem] 1999 Jan; Vol. 125 (1), pp. 160-5. - Publication Year :
- 1999
-
Abstract
- This paper describes a new HPLC method for the sugar analysis of algal galactans containing 3,6-anhydrogalactose, which is readily destroyed during usual acid hydrolysis and methanolysis. By anhydrous mercaptolysis of galactans at 60 degreesC for 6 h with the newly developed solvent system, 0.5 N HCl/[ethanethiol:methanol (2:1, v/v)], component sugars, including 3,6-anhydrogalactose, were liberated quantitatively as their diethyl dithioacetals. The resultant sugar diethyl dithioacetals were found to have strong UV-absorption (absorption maximum of 3,6-anhydrogalactose diethyl dithioacetal in water, 191-192 nm; molar extinction coefficient, 4,400). The sugar diethyl dithioacetals were then resolved simultaneously within 15 min by reversed phase HPLC with 30% acetonitrile as the eluent and detected by UV-absorption at 215 nm. Amounts of sugar diethyl dithioacetals less than 50 pmol can be determined without further derivatization.
- Subjects :
- Absorption
Galactans chemistry
Galactose analogs & derivatives
Galactose analysis
Galactose chemistry
Spectrophotometry, Ultraviolet
Sulfhydryl Compounds chemistry
Biochemistry methods
Carbohydrates analysis
Chromatography, High Pressure Liquid methods
Eukaryota chemistry
Galactans analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0021-924X
- Volume :
- 125
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 9880812
- Full Text :
- https://doi.org/10.1093/oxfordjournals.jbchem.a022253