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Monoclonal antibody recognizing a core epitope on mucin.
- Source :
-
Disease markers [Dis Markers] 1998 Oct; Vol. 14 (2), pp. 99-112. - Publication Year :
- 1998
-
Abstract
- Monoclonal antibody TH1 (IgM) was prepared by immunizing mice with deglycosylated (TFMSA-treated) cystic fibrosis mucin. TH1 reacted strongly with TFMSA treated cystic fibrosis mucin but not with the fully glycosylated mucin, indicating reactivity with a core mucin epitope. TH1 showed no reactivity with ovine mucin (98% of glycans as sialyl-Tn) but reacted strongly with desialylated ovine mucin, indicating the epitope for this mab was the Tn-antigen (O-linked GalNAc). However, TH1 showed no reactivity with Tn-positive red blood cells, and the binding of TH1 was not inhibited by GalNAc at 2.5 mg/ml, illustrating the importance of the peptide sequence to which the GalNAc is attached. TH1 stained the majority of cancers of the colon, lung, stomach, ovary, breast, and cervix, and the cellular distribution of this antigen in normal tissue suggested reactivity with immature mucin. This antibody appears to be a useful reagent for the detection of immature mucin.
- Subjects :
- Amino Acid Sequence
Animals
Antibodies, Monoclonal immunology
Antibody Specificity
Breast Neoplasms metabolism
Colonic Neoplasms metabolism
Cystic Fibrosis metabolism
Enzyme-Linked Immunosorbent Assay
Female
Glycosylation
Hybridomas immunology
Immunoenzyme Techniques
Mesylates pharmacology
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Mucins analysis
Mucins chemistry
Neoplasms chemistry
Ovarian Neoplasms metabolism
Tumor Cells, Cultured
Antibodies, Monoclonal biosynthesis
Epitopes immunology
Mucins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0278-0240
- Volume :
- 14
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Disease markers
- Publication Type :
- Academic Journal
- Accession number :
- 9868597
- Full Text :
- https://doi.org/10.1155/1998/678434