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Mucin-related epitopes distinguish M cells and enterocytes in rabbit appendix and Peyer's patches.
- Source :
-
Infection and immunity [Infect Immun] 1999 Jan; Vol. 67 (1), pp. 357-67. - Publication Year :
- 1999
-
Abstract
- The biochemical composition of the apical membranes of epithelial M cells overlying the gut-associated lymphoid tissues (GALT) is still largely unknown. We have prepared monoclonal antibodies (MAbs) directed against carbonate-washed plasma membranes from epithelial cells detached with EDTA from rabbit appendix, a tissue particularly rich in GALT. As determined by immunofluorescence microscopy, several MAbs specifically recognized either M cells or enterocyte-like cells of the domes from rabbit appendix, sacculus rotundus, and Peyer's patches. M cells were identified by their large ventral pocket containing lymphoid cells and by specific labeling with antivimentin. Among various characterized MAbs, MAb 104 recognized rabbit immunoglobulins and was used as an apical marker for M cells in the rabbit appendix, MAb 58 selectively stained an integral membrane glycoprotein of greater than 205 kDa located at the apex of M cells, and MAb 214 stained a smaller soluble glycoprotein associated with the apical surfaces from neighboring enterocytes. In addition, both MAbs 58 and 214 also labeled luminal mucus and secretory granules in goblet cells. The selective association of mucin-related molecules at the surfaces of either M cells or enterocyte-like cells of the follicle-associated epithelium suggests that specific carbohydrate antigens are differentially expressed by epithelial cells and could account for the differential binding properties of pathogens.
- Subjects :
- Animals
Antibodies, Monoclonal biosynthesis
Antigens, Bacterial chemistry
Antigens, Bacterial ultrastructure
Appendix chemistry
Appendix ultrastructure
Bacterial Proteins chemistry
Bacterial Proteins ultrastructure
Cell Line
Cell Membrane immunology
Cell Separation methods
Edetic Acid
Epithelial Cells chemistry
Epithelial Cells ultrastructure
Epitopes biosynthesis
Epitopes ultrastructure
Female
Gerbillinae
Immunoglobulin A analysis
Intestinal Mucosa chemistry
Intestinal Mucosa ultrastructure
Mice
Mice, Inbred BALB C
Peyer's Patches chemistry
Peyer's Patches ultrastructure
Rabbits
Appendix immunology
Epithelial Cells immunology
Epitopes analysis
Intestinal Mucosa immunology
Mucins immunology
Peyer's Patches immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 67
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 9864237
- Full Text :
- https://doi.org/10.1128/IAI.67.1.357-367.1999