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Insulin-like growth factor I plays a role in gastric wound healing: evidence using a zinc derivative, polaprezinc, and an in vitro rabbit wound repair model.
- Source :
-
Alimentary pharmacology & therapeutics [Aliment Pharmacol Ther] 1998 Nov; Vol. 12 (11), pp. 1131-8. - Publication Year :
- 1998
-
Abstract
- Background: Although the detailed mechanism is unclear, zinc and its derivative, polaprezinc, have been reported to accelerate gastric ulcer healing in vivo.<br />Aim: To investigate the detailed cellular mechanism of polaprezinc on gastric epithelial cells and fibroblasts with special attention to insulin-like growth factor I (IGF-I).<br />Methods: Isolated rabbit gastric epithelial cells formed a complete monolayer, from which a circular artificial wound with constant size was made. The restoration process was monitored by measuring wound size up to 48 h. Either polaprezinc, IGF-I, fibroblast conditioned medium or neutralized medium conditioned by anti-IGF-I antibody was added at the time of wounding. The expression of mRNA of IGF-I, hepatocyte growth factor (HGF) and transforming growth factor alpha (TGF-alpha) in fibroblasts with or without polaprezinc treatment was tested using reverse transcription polymerase chain reaction (RT-PCR). Gastric epithelial cell proliferation was also examined by bromodeoxyuridine (BrdU) staining.<br />Results: IGF-I and fibroblast conditioned medium treatment accelerated gastric epithelial restoration which included cell migration and proliferation. However, polaprezinc and neutralized conditioned medium treatment did not accelerate epithelial repair. RT-PCR for growth factor mRNA revealed the IGF-I mRNA expression in fibroblasts was increased after treatment with polaprezinc.<br />Conclusion: Polaprezinc induced IGF-I production from mesenchymal cells, resulting in stimulation of epithelial cell restoration through a paracrine pathway. IGF-I may play an important role in gastric wound repair.
- Subjects :
- Animals
Blotting, Southern
Carnosine pharmacology
Cell Division drug effects
Cell Movement drug effects
Cells, Cultured
Fibroblasts metabolism
Gastric Mucosa cytology
Gene Expression
Growth Substances analysis
Insulin-Like Growth Factor I genetics
Male
Mesoderm
RNA, Messenger metabolism
Rabbits
Reverse Transcriptase Polymerase Chain Reaction
Zinc Compounds
Carnosine analogs & derivatives
Gastric Mucosa drug effects
Insulin-Like Growth Factor I pharmacology
Organometallic Compounds pharmacology
Wound Healing drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0269-2813
- Volume :
- 12
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Alimentary pharmacology & therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 9845403
- Full Text :
- https://doi.org/10.1046/j.1365-2036.1998.00408.x