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A 500-kb YAC and BAC contig encompassing the high-growth deletion in mouse chromosome 10 and identification of the murine Raidd/Cradd gene in the candidate region.
- Source :
-
Genomics [Genomics] 1998 Nov 15; Vol. 54 (1), pp. 159-64. - Publication Year :
- 1998
-
Abstract
- The mouse high growth (hg) gene was identified in a selection experiment for rapid growth. It produces a 30-50% increase in weight gain of homozygous individuals without resulting in obesity. Recently, hg was mapped to a deletion around marker D10Mit69. Here we report a map of yeast artificial chromosome and bacterial artificial chromosome clones spanning the entire region deleted in high-growth mice. The size of the deletion estimated from the clone lengths is approximately 500 kb. Using exon trapping, the murine Raidd/Cradd gene was identified in the hg region, and its cDNA sequence and expression pattern were determined. The human RAIDD/CRADD was mapped using radiation hybrid mapping to human chromosome 12, 2.9 cR distal to marker AFMB311WC5. The identified Raidd/Cradd gene, which is deleted in high-growth mice, presents a potential candidate for hg.<br /> (Copyright 1998 Academic Press.)
- Subjects :
- Amino Acid Sequence
Animals
Blotting, Northern
CRADD Signaling Adaptor Protein
Carrier Proteins chemistry
Carrier Proteins metabolism
Cloning, Molecular
DNA, Complementary
Gene Deletion
Genetic Markers
Humans
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Polymerase Chain Reaction
Proteins chemistry
Proteins metabolism
Sequence Tagged Sites
Adaptor Proteins, Signal Transducing
Carrier Proteins genetics
Contig Mapping
Growth genetics
Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0888-7543
- Volume :
- 54
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 9806843
- Full Text :
- https://doi.org/10.1006/geno.1998.5540