Back to Search
Start Over
Areca nut extract inhibits the growth, attachment, and matrix protein synthesis of cultured human gingival fibroblasts.
- Source :
-
Journal of periodontology [J Periodontol] 1998 Oct; Vol. 69 (10), pp. 1092-7. - Publication Year :
- 1998
-
Abstract
- Betel quid chewing is a popular oral habit in India, South Africa, and many Southeast Asian countries. The effects of areca nut (AN) extract on the growth, attachment, and protein synthesis of healthy human gingival fibroblasts (GF) were investigated to determine why betel quid (BQ) chewers have higher prevalence of periodontal disease than non-chewers. Twenty-four hour exposure of human GF to AN extract (> 200 microg/ml) in culture led to the formation of numerous intracellular vacuoles. As analyzed by modified MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] assay, AN extract significantly suppressed the growth of GF over 5 days of incubation in a dose-dependent manner. At concentrations of 50 and 300 microg/ml, AN extract suppressed the growth of GF with 30% and 57% (P < 0.05), respectively. AN extract also significantly suppressed the synthesis of [3H]proline incorporation into trichloroacetic acid (TCA) precipitated proteins. At concentrations of 200, 400, and 600 microg/ml, AN extract suppressed the protein synthesis with 33%, 58%, and 63% of inhibition (P < 0.05), respectively. Preincubation of cells in a medium containing AN extract for 2 hours inhibits the subsequent attachment of cultured GF to type I collagen at the 50% inhibitory concentration (IC50) which is about 720 to 798 microg/ml. Considering the frequent consumption of BQ throughout the day, impairment of sequential fibroblast functions by BQ ingredients is a potential mechanism through which BQ chewing exert a deleterious effect to the gingival tissues.
- Subjects :
- Cell Adhesion drug effects
Cell Division drug effects
Cells, Cultured
Collagen chemistry
Coloring Agents
Dose-Response Relationship, Drug
Extracellular Matrix Proteins antagonists & inhibitors
Extracellular Matrix Proteins biosynthesis
Fibroblasts cytology
Fibroblasts metabolism
Gingiva cytology
Gingiva metabolism
Humans
Periodontal Diseases etiology
Plant Extracts administration & dosage
Prevalence
Proline metabolism
Radiopharmaceuticals
Tetrazolium Salts
Thiazoles
Time Factors
Tritium
Vacuoles drug effects
Vacuoles ultrastructure
Areca adverse effects
Extracellular Matrix Proteins drug effects
Fibroblasts drug effects
Gingiva drug effects
Plant Extracts pharmacology
Plants, Medicinal
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3492
- Volume :
- 69
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of periodontology
- Publication Type :
- Academic Journal
- Accession number :
- 9802706
- Full Text :
- https://doi.org/10.1902/jop.1998.69.10.1092