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Involvement of CD14 on human gingival fibroblasts in Porphyromonas gingivalis lipopolysaccharide-mediated interleukin-6 secretion.
- Source :
-
Archives of oral biology [Arch Oral Biol] 1998 Sep; Vol. 43 (9), pp. 687-94. - Publication Year :
- 1998
-
Abstract
- The lipopolysaccharides (LPS) of Porphyromonas gingivalis are implicated in the initiation and development of periodontal diseases. However, the mechanisms underlying P. gingivalis LPS-mediated periodontal destruction are still unknown. Here, it was found that P. gingivalis LPS activates human gingival fibroblasts (HGF) to release interleukin 6 (IL-6) via CD14. Flow-cytometric analysis showed that HGFs bind to fluorescein-isothiocyanate (FITC)-labelled LPS, and express CD14 on their surfaces. The binding of FITC LPS was competitively suppressed by unlabelled synthetic lipid A as well as by LPS. LPS-induced IL-6 production was inhibited by anti-CD14 monoclonal antibody in a dose-dependent manner. The binding of FITC LPS to HGF was abrogated by anti-CD14 monoclonal antibody. Engagement of LPS initiated the protein tyrosine phosphorylation of several intracellular proteins including extracellular signal-regulated kinase (ERK) 1 and 2, and these events were suppressed by the anti-CD14 monoclonal. These results suggest that CD14 is a cell surface binding site for LPS and is involved in the LPS-mediated activation of HGF.
- Subjects :
- Antibodies, Monoclonal
Binding Sites
Binding, Competitive
Blotting, Western
Calcium-Calmodulin-Dependent Protein Kinases metabolism
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Gingiva cytology
Humans
Lipid A immunology
Mitogen-Activated Protein Kinase 1 metabolism
Mitogen-Activated Protein Kinase 3
Phosphorylation
Precipitin Tests
Tyrosine metabolism
Fibroblasts immunology
Gingiva immunology
Interleukin-6 metabolism
Lipopolysaccharide Receptors immunology
Lipopolysaccharides immunology
Mitogen-Activated Protein Kinases
Porphyromonas gingivalis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0003-9969
- Volume :
- 43
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Archives of oral biology
- Publication Type :
- Academic Journal
- Accession number :
- 9783822
- Full Text :
- https://doi.org/10.1016/s0003-9969(98)00056-9