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Immunophenotyping and T-cell receptor gamma gene rearrangement analysis as an adjunct to the histopathologic diagnosis of mycosis fungoides.
- Source :
-
Journal of the American Academy of Dermatology [J Am Acad Dermatol] 1998 Oct; Vol. 39 (4 Pt 1), pp. 554-9. - Publication Year :
- 1998
-
Abstract
- Background: The histopathologic diagnosis of mycosis fungoides (MF) may be difficult.<br />Objective: Our purpose was to evaluate the role of immunophenotyping and T-cell receptor (TCR) gene rearrangement studies as an adjunct to the histopathologic diagnosis of MF.<br />Methods: Immunohistochemical studies with antibodies to CD4, CD5, CD7, and CD8 and TCR gamma gene rearrangement analysis with a polymerase chain reaction were performed on fresh-frozen material of patients with "classic" histology of MF, "inconclusive" histology, and benign inflammatory dermatoses.<br />Results: Clonal TCR gamma gene rearrangements were found in 11 of 16 (69%) of classic MF cases, in 3 of 19 (16%) of inconclusive cases, and in none of the 12 inflammatory dermatoses cases (P < .05 and P < .001, respectively). Only the mean CD7 counts were statistically significantly different between these 3 groups (MF < inconclusive < inflammatory).<br />Conclusion: Inconclusive histology is probably a heterogeneous group in which CD7 counts and TCR gamma gene rearrangement studies might help to differentiate the MF cases from the benign cases.
- Subjects :
- Adolescent
Adult
Aged
Child
Diagnosis, Differential
Female
Humans
Immunohistochemistry
Immunophenotyping
Male
Middle Aged
Mycosis Fungoides immunology
Polymerase Chain Reaction
Skin Neoplasms immunology
Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
Genes, T-Cell Receptor gamma
Mycosis Fungoides diagnosis
Mycosis Fungoides genetics
Skin Neoplasms diagnosis
Skin Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0190-9622
- Volume :
- 39
- Issue :
- 4 Pt 1
- Database :
- MEDLINE
- Journal :
- Journal of the American Academy of Dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 9777761
- Full Text :
- https://doi.org/10.1016/s0190-9622(98)70003-9