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Unique biochemical properties of human leukocyte antigen-E allow for a highly specific function in immune recognition.
- Source :
-
American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 1998 Sep; Vol. 40 (3), pp. 177-82. - Publication Year :
- 1998
-
Abstract
- Problem: Does a correlation exist between the expression of human leukocyte antigen (HLA) class Ia and HLA-E and what is its biological significance?<br />Method of Study: HLA-E transcripts were detected by reverse transcriptase-polymerase chain reaction. Metabolically labeled HLA-E heavy chains were immunoprecipited and analyzed by one-dimensional isoelectric focusing. Mouse RMA-S cells defective with regard to transporter associated with antigen processing (TAP) function were transfected with HLA-E and human beta 2-microglobulin to investigate TAP dependence of the cell-surface expression of HLA-E.<br />Results: HLA-E is transcribed regardless of the down-regulation of polymorphic HLA class Ia expression. HLA-E is transported to the cell surface in the absence of TAP-controlled peptide loading. In human cells, the amount of HLA-E protein is very low regardless of the presence of correct peptide ligands.<br />Conclusions: HLA-E regulates immune functions in cells that have down-regulated the expression of polymorphic HLA-class Ia molecules, either by preventing harmful natural killer cells from attacking targets that have physiologically decreased HLA-class Ia expression or by activating effector cells against virus-infected and tumor cells with impaired HLA-class Ia expression.
- Subjects :
- Animals
Antibodies, Monoclonal
HLA Antigens biosynthesis
HLA Antigens genetics
Histocompatibility Antigens Class I biosynthesis
Histocompatibility Antigens Class I genetics
Humans
Isoelectric Focusing
Mice
Precipitin Tests
RNA, Messenger analysis
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Transfection
HLA-E Antigens
HLA Antigens immunology
Histocompatibility Antigens Class I immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1046-7408
- Volume :
- 40
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of reproductive immunology (New York, N.Y. : 1989)
- Publication Type :
- Academic Journal
- Accession number :
- 9764362
- Full Text :
- https://doi.org/10.1111/j.1600-0897.1998.tb00410.x