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Effect of interleukin-8 on production of tumor-associated substances and autocrine growth of human liver and pancreatic cancer cells.
- Source :
-
Cancer immunology, immunotherapy : CII [Cancer Immunol Immunother] 1998 Sep; Vol. 47 (1), pp. 47-57. - Publication Year :
- 1998
-
Abstract
- We have previously reported that human liver cancer cell lines produce interleukin-8 (IL-8) at high levels. Those tumor cells appeared to express two kinds of IL-8 receptor on their surface. In order to analyze the role of IL-8 on the biological characteristics of those tumor cells, we suppressed IL-8 production from human liver (HuH-7 and HuCC-T1) and pancreatic cancer cell lines (HuP-T4) by treatment with IL-8 antisense oligonucleotides. Suppression of IL-8 production resulted not only in inhibition of cell growth, but also in an increase in the concentrations of some tumor-associated substances such as carbohydrate antigen 19-9 (CA19-9) in the medium. These data indicate that IL-8 produced by human liver and pancreatic tumors may act as an autocrine growth factor and may control the production of some tumor-associated substances. Furthermore, surface expression of sialyl-Lewis(a), which is a ligand for ELAM-1 on human umbilical vein endothelial cells (HUVEC), HuCC-T1 and HuP-T4 cells was decreased and the attachment of these tumor cells to HUVEC was inhibited by treatment with IL-8 antisense oligonucleotide. Since the soluble form of CA19-9 (sialyl-Lewis(a)) was shown to inhibit the tumor cell binding to HUVEC, the decrease in release of CA19-9 into the medium and increase in the expression of sialyl-Lewis(a) on the cell surface may suggest that IL-8 production from the tumor cells enhances metastatic potential by augmenting the binding activity of the tumor cells to HUVEC. These data demonstrate that a cytokine produced by tumor cells may function as an autocrine growth factor and affect tumor cell dissemination.
- Subjects :
- Antigens, CD biosynthesis
Cell Adhesion drug effects
Cell Division drug effects
Cell Transformation, Neoplastic drug effects
Cytokines metabolism
Endothelium, Vascular cytology
Humans
Interleukin-1 pharmacology
Interleukin-8 antagonists & inhibitors
Interleukin-8 metabolism
Membrane Proteins biosynthesis
Neoplasm Proteins metabolism
Oligonucleotides, Antisense pharmacology
Pancreatic Neoplasms pathology
Receptors, Chemokine biosynthesis
Receptors, Cytokine biosynthesis
Receptors, Interleukin biosynthesis
Receptors, Interleukin-8A
Receptors, Interleukin-8B
Recombinant Proteins pharmacology
Tumor Cells, Cultured metabolism
Umbilical Veins cytology
Interleukin-8 pharmacology
Liver Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0340-7004
- Volume :
- 47
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cancer immunology, immunotherapy : CII
- Publication Type :
- Academic Journal
- Accession number :
- 9755878
- Full Text :
- https://doi.org/10.1007/s002620050503