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TGF-beta2 inhibits growth of uveal melanocytes at physiological concentrations.

Authors :
Hu DN
McCormick SA
Lin AY
Lin JY
Source :
Experimental eye research [Exp Eye Res] 1998 Aug; Vol. 67 (2), pp. 143-50.
Publication Year :
1998

Abstract

The effect of TGF-beta2 on growth of uveal melanocytes in vitro was studied and the dose-dependent inhibitory effect of TGF-beta2 was compared with the known concentration of TGF-beta2 in aqueous humor. Uveal melanocytes were isolated and cultured with medium supplemented with cAMP elevating agents and basic fibroblast growth factor. The uveal melanocytes were plated into multi-well plates. After 24 hr, TGF-beta2 was added to the medium in various concentrations. After 5 days, the cells were detached, counted and compared to the controls. The effect of TGF-beta2 on DNA synthesis (as evaluated by uptake of bromodeoxyuridine) were also tested. TGF-beta2 inhibited growth and DNA synthesis of cultured uveal melanocytes in a dose-dependent manner at concentrations from 0.03-10.0 ng ml-1. The growth-inhibition of TGF-beta2 was present even in serum-free medium. TGF-beta2 had little or no effect on melanogenesis of cultured uveal melanocytes. The serum used for cultivation did not contain active TGF-beta1 or TGF-beta2 as measured by immunoassay. The known amount of active TGF-beta2 in aqueous humor (0.2-0.4 ng ml-1) is sufficient to inhibit the growth of uveal melanocytes. It indicates that TGF-beta2 is a potent growth inhibit factor of uveal melanocytes and may play an important role in maintaining the non-proliferative, relatively quiescence status of uveal melanocytes in vivo.<br /> (Copyright 1998 Academic Press)

Details

Language :
English
ISSN :
0014-4835
Volume :
67
Issue :
2
Database :
MEDLINE
Journal :
Experimental eye research
Publication Type :
Academic Journal
Accession number :
9733581
Full Text :
https://doi.org/10.1006/exer.1998.0501