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Shh, Bmp-2 and Hoxd-13 gene expression in chick limb bud cells in culture.
- Source :
-
Development, growth & differentiation [Dev Growth Differ] 1998 Aug; Vol. 40 (4), pp. 457-64. - Publication Year :
- 1998
-
Abstract
- The anteroposterior axis of the vertebrate limb bud is determined by signals from the zone of polarizing activity (ZPA). Sonic hedgehog (Shh) is expressed in the posterior mesoderm, which corresponds closely to ZPA activity. Moreover, Bmp-2 and HoxD genes are expressed in the broader posterior mesoderm, and it is thought that the ZPA signaling pathway consists of these gene products. Limb outgrowth and patterning, including expression of these genes, depend on the apical ectodermal ridge (AER). Fibroblast growth factors (FGF) have been identified as candidates for signal molecules from the AER. To further understand the ZPA signaling pathway and the participation of FGF, expressions of these genes were examined by reverse transcription-polymerase chain reaction in chick limb bud cells cultured with FGF-4. The present results indicate that FGF-4 cannot maintain Shh expression but can maintain Hoxd-13 expression in cultured posterior cells; moreover, Bmp-2 is expressed independently of FGF-4. These results suggest that Bmp-2 and Hoxd-13 expressions do not require a continuous expression of Shh. Further, it was demonstrated that posterior cells cultured with FGF-4 recovered Shh expression when grafted to the limb bud, indicating that FGF-4 maintains not Shh expression itself but competence of Shh expression.
- Subjects :
- Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins metabolism
Bone Morphogenetic Proteins physiology
Chick Embryo
Culture Techniques
Egg Proteins analysis
Egg Proteins metabolism
Embryonic Induction physiology
Fibroblast Growth Factor 4
Fibroblast Growth Factors pharmacology
Gene Expression Regulation, Developmental
Hedgehog Proteins
Homeodomain Proteins metabolism
Homeodomain Proteins physiology
Limb Buds embryology
Membrane Glycoproteins analysis
Membrane Glycoproteins metabolism
Mesoderm drug effects
Mesoderm metabolism
Mesoderm transplantation
Proteins metabolism
Proteins physiology
Proto-Oncogene Proteins pharmacology
Signal Transduction genetics
Signal Transduction physiology
Transforming Growth Factor beta metabolism
Transforming Growth Factor beta physiology
Zona Pellucida Glycoproteins
Bone Morphogenetic Proteins genetics
Embryonic Induction genetics
Homeodomain Proteins genetics
Limb Buds metabolism
Proteins genetics
Receptors, Cell Surface
Trans-Activators
Transcription Factors
Transforming Growth Factor beta genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0012-1592
- Volume :
- 40
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Development, growth & differentiation
- Publication Type :
- Academic Journal
- Accession number :
- 9727360
- Full Text :
- https://doi.org/10.1046/j.1440-169x.1998.t01-2-00011.x