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Molecular cloning and characterization of a cDNA encoding mouse phospholipase C-beta3.
Molecular cloning and characterization of a cDNA encoding mouse phospholipase C-beta3.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1998 Jul 31; Vol. 1393 (1), pp. 173-8. - Publication Year :
- 1998
-
Abstract
- A cDNA encoding mouse PLC-beta3 (mPLC-beta3) was identified by screening a mouse kidney cDNA library and using the rapid amplification of cDNA ends (RACE) method. The predicted open reading frame was 3705 bp in length. The deduced 1235 amino acid (aa) sequence shares 95.3% and 92% homology with the sequences of rat and human PLC-beta3, respectively. The corresponding mRNA is highly expressed in kidney, skeletal muscle, liver, lung, heart and brain. In spleen, mPLC-beta3 mRNA was not detectable, which is in contrast to humans where there is a distinct expression. Using ultrastructural immunocytochemistry, mPLC-beta3 expression was detected in the heterochromatin of the nucleus in mouse brain neurons. The observation of PLC-beta3 nuclear localization suggests that PLC-beta3 may have intranuclear functions.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Brain ultrastructure
Cell Nucleus enzymology
Cloning, Molecular
Gene Library
Heterochromatin ultrastructure
Immunohistochemistry
Isoenzymes analysis
Isoenzymes chemistry
Mice
Molecular Sequence Data
Phospholipase C beta
Sequence Homology, Amino Acid
Type C Phospholipases analysis
Type C Phospholipases chemistry
Brain enzymology
DNA, Complementary chemistry
Isoenzymes genetics
Type C Phospholipases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 1393
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 9714794
- Full Text :
- https://doi.org/10.1016/s0005-2760(98)00074-5