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Three-dimensional 87Rb imaging of isolated pig hearts: effects of regional ischemia.
- Source :
-
Magnetic resonance in medicine [Magn Reson Med] 1998 Aug; Vol. 40 (2), pp. 175-9. - Publication Year :
- 1998
-
Abstract
- The aim of this work was to investigate whether 1) Rb uptake is reduced in ischemic myocardium, and 2) 87Rb three-dimensional (3D) imaging can detect the ischemic area. Hearts of domestic pigs (n = 8, 20-30 kg) were perfused retrogradely with Krebs-Henseleit buffer in a 7-T, 40-cm horizontal bore magnet interfaced with Bruker MSLX spectrometer. Control (C) and ischemic (I) (45 min ligation of the left anterior descending coronary artery (LAD)) hearts were loaded with Rb+ by perfusion with a Rb(+)-containing solution (2-4.7 mM, 30-100% of K+ substitution) for 35 min and 87Rb (C) or 31P (ischemic area in I) spectra were acquired. After mechanical arrest with 0.6-0.9 mM lidocaine, 87Rb images (7 min each, 1 cm3 resolution) were acquired (30-40 min) in the presence of Rb(+)-containing perfusate. Subsequently, the hearts were stained with Evans blue (EB) and samples taken for measurements of Rb+ content. In the Group C, distribution of Rb+ in the left ventricle and the intensities of the 3D 87Rb images were uniform. In the ischemic area (Group I), verified by the lack of EB staining and changes in 31P spectra, the images showed a reduced intensity, which corresponded to decreased Rb+ content (33 +/- 11% of the normal). Thus, 87Rb imaging reveals damaged cells detecting reduced Rb+ content in the ischemic area.
- Subjects :
- Animals
Myocardial Ischemia pathology
Necrosis
Phosphates metabolism
Potassium metabolism
Sodium-Potassium-Exchanging ATPase metabolism
Swine
Tissue Survival physiology
Image Processing, Computer-Assisted
Magnetic Resonance Imaging
Magnetic Resonance Spectroscopy
Myocardial Ischemia diagnosis
Rubidium Radioisotopes pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 0740-3194
- Volume :
- 40
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Magnetic resonance in medicine
- Publication Type :
- Academic Journal
- Accession number :
- 9702698
- Full Text :
- https://doi.org/10.1002/mrm.1910400202