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Transcription factor B-cell-specific activator protein (BSAP) is differentially expressed in B cells and in subsets of B-cell lymphomas.
- Source :
-
Blood [Blood] 1998 Aug 15; Vol. 92 (4), pp. 1308-16. - Publication Year :
- 1998
-
Abstract
- The paired box containing gene PAX-5 encodes the transcription factor BSAP (B-cell-specific activator protein), which plays a key role in B-lymphocyte development. Despite its known involvement in a rare subtype of non-Hodgkin's lymphoma (NHL), a detailed examination of BSAP expression in NHL has not been previously reported. In this study, we analyzed normal and malignant lymphoid tissues and cell lines, including 102 cases of B-cell NHL, 23 cases of T- and null-cell NHL, and 18 cases of Hodgkin's disease. Normal lymphoid tissues showed strong nuclear BSAP expression in mantle zone B cells, less intense reactivity in follicular center B cells, and no expression in cells of the T-cell-rich zones. Monocytoid B cells showed weak expression, whereas plasma cells and extrafollicular large transformed B cells were negative. Of the 102 B-cell NHLs, 83 (81%) demonstrated BSAP expression. All of the 13 (100%) B-cell chronic lymphocytic leukemias (B-CLLs), 21 of (100%) mantle cells (MCLs), and 20 of 21 (95%) follicular lymphomas (FLs) were positive. Moderate staining intensities were found in most B-CLL and FL cases, whereas most MCLs showed strong reactions, paralleling the strong reactivity of nonmalignant mantle cells. Eight of 12 (67%) marginal zone lymphoma cases showed negative or low BSAP levels, and 17 of 24 (71%) large B-cell lymphomas displayed moderate to strong expression. None of the 23 T- and null-cell lymphomas reacted with the BSAP antisera, whereas in Hodgkin's disease, 2 of 4 (50%) nodular lymphocytic predominance and 5 of 14 (36%) classical cases showed weak nuclear or nucleolar BSAP reactions in a fraction of the tumor cells. Western blot analysis showed a 52-kD BSAP band in B-cell lines, but not in non-B-cell or plasma cell lines. We conclude that BSAP expression is largely restricted to lymphomas of B-cell lineage and that BSAP expression varies in B-cell subsets and subtypes of B-cell NHL. The high levels of BSAP, especially those found in large-cell lymphomas and in some follicular lymphomas, may be a consequence of deregulated gene expression and suggest a possible involvement of PAX-5 in certain B-cell malignancies. This is a US government work. There are no restrictions on its use.
- Subjects :
- Antigens, CD20 analysis
Biomarkers, Tumor analysis
Cell Lineage
Cell Transformation, Neoplastic genetics
DNA-Binding Proteins genetics
Gene Expression Regulation, Neoplastic
Genes, Homeobox
Hematologic Neoplasms metabolism
Hematologic Neoplasms pathology
Hodgkin Disease metabolism
Hodgkin Disease pathology
Humans
Leukemia, Lymphocytic, Chronic, B-Cell pathology
Lymphoma, B-Cell classification
Lymphoma, B-Cell pathology
Lymphoma, Follicular metabolism
Lymphoma, Follicular pathology
Lymphoma, Large B-Cell, Diffuse metabolism
Lymphoma, Large B-Cell, Diffuse pathology
Lymphoma, T-Cell metabolism
Lymphoma, T-Cell pathology
Neoplasm Proteins genetics
Nuclear Proteins genetics
PAX5 Transcription Factor
Transcription Factors genetics
Tumor Cells, Cultured
B-Lymphocyte Subsets metabolism
DNA-Binding Proteins biosynthesis
Gene Expression Regulation
Leukemia, Lymphocytic, Chronic, B-Cell metabolism
Lymphoma, B-Cell metabolism
Neoplasm Proteins biosynthesis
Neoplastic Stem Cells metabolism
Nuclear Proteins biosynthesis
Transcription Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 92
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 9694719