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Stimulatory effect of lithium on glucose transport in rat adipocytes is not mediated by elevation of IP1.
- Source :
-
The American journal of physiology [Am J Physiol] 1998 Aug; Vol. 275 (2), pp. E272-7. - Publication Year :
- 1998
-
Abstract
- Lithium has been shown to increase glucose uptake in skeletal muscle and adipose tissues. The therapeutic effect of lithium on bipolar disease is thought to be mediated by its inhibitory effect on myo-inositol-1-monophosphatase (IMPase). We tested the hypothesis that the stimulatory effect of lithium on glucose uptake results from inhibition of IMPase and the resultant accumulation of inositol monophosphates (IP1) by comparing the effects of lithium and a selective IMPase inhibitor, L-690,488, on isolated rat adipocytes. Insulin produced a concentration-dependent stimulation of 2-deoxy-D-[14C]glucose (2-DG) transport (10 microU/ml caused half-maximal activation). Acute exposure to lithium stimulated basal glucose transport activity in a concentration-dependent manner, with a threefold stimulation at 30 mM lithium. Lithium also potentiated insulin-stimulated 2-DG transport. Lithium produced a concomitant increase in IP1 accumulation. In contrast, L-690,488 increased IP1 to levels comparable to those of lithium without stimulatory effects on 2-DG transport. These results demonstrate that stimulatory effects of lithium on glucose transport are not mediated by the inhibition of IMPase and subsequent accumulation of IP1 in rat adipocytes.
- Subjects :
- Adipocytes drug effects
Adipose Tissue metabolism
Animals
Biological Transport drug effects
Cells, Cultured
Diphosphonates pharmacology
Enzyme Inhibitors pharmacology
Epididymis
Inositol metabolism
Insulin pharmacology
Kinetics
Male
Phosphoric Monoester Hydrolases antagonists & inhibitors
Phosphoric Monoester Hydrolases metabolism
Prodrugs pharmacology
Rats
Rats, Sprague-Dawley
Adipocytes metabolism
Deoxyglucose metabolism
Glucose metabolism
Inosine Monophosphate metabolism
Lithium Chloride pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 275
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 9688629
- Full Text :
- https://doi.org/10.1152/ajpendo.1998.275.2.E272