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Use of the lactococcal nisA promoter to regulate gene expression in gram-positive bacteria: comparison of induction level and promoter strength.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 1998 Aug; Vol. 64 (8), pp. 2763-9. - Publication Year :
- 1998
-
Abstract
- We characterized the regulated activity of the lactococcal nisA promoter in strains of the gram-positive species Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus pneumoniae, Enterococcus faecalis, and Bacillus subtilis. nisA promoter activity was dependent on the proteins NisR and NisK, which constitute a two-component signal transduction system that responds to the extracellular inducer nisin. The nisin sensitivity and inducer concentration required for maximal induction varied among the strains. Significant induction of the nisA promoter (10- to 60-fold induction) was obtained in all of the species studied at a nisin concentration just below the concentration at which growth is inhibited. The efficiency of the nisA promoter was compared to the efficiencies of the Spac, xylA, and lacA promoters in B. subtilis and in S. pyogenes. Because nisA promoter-driven expression is regulated in many gram-positive bacteria, we expect it to be useful for genetic studies, especially studies with pathogenic streptococci in which no other regulated promoters have been described.
- Subjects :
- Bacillus subtilis drug effects
Bacterial Proteins physiology
Base Sequence
Dose-Response Relationship, Drug
Glucuronidase metabolism
Microbial Sensitivity Tests
Nisin genetics
Nisin metabolism
Nisin pharmacology
Plasmids genetics
Signal Transduction
Streptococcaceae drug effects
Bacillus subtilis genetics
Gene Expression Regulation, Bacterial
Lactococcus lactis genetics
Promoter Regions, Genetic
Streptococcaceae genetics
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 64
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 9687428
- Full Text :
- https://doi.org/10.1128/AEM.64.8.2763-2769.1998