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The cytosolic DnaJ-like protein djp1p is involved specifically in peroxisomal protein import.
- Source :
-
The Journal of cell biology [J Cell Biol] 1998 Jul 27; Vol. 142 (2), pp. 421-34. - Publication Year :
- 1998
-
Abstract
- The Saccharomyces cerevisiae DJP1 gene encodes a cytosolic protein homologous to Escherichia coli DnaJ. DnaJ homologues act in conjunction with molecular chaperones of the Hsp70 protein family in a variety of cellular processes. Cells with a DJP1 gene deletion are viable and exhibit a novel phenotype among cytosolic J-protein mutants in that they have a specific impairment of only one organelle, the peroxisome. The phenotype was also unique among peroxisome assembly mutants: peroxisomal matrix proteins were mislocalized to the cytoplasm to a varying extent, and peroxisomal structures failed to grow to full size and exhibited a broad range of buoyant densities. Import of marker proteins for the endoplasmic reticulum, nucleus, and mitochondria was normal. Furthermore, the metabolic adaptation to a change in carbon source, a complex multistep process, was unaffected in a DJP1 gene deletion mutant. We conclude that Djp1p is specifically required for peroxisomal protein import.
- Subjects :
- Amino Acid Sequence
Base Sequence
Biological Transport, Active
Cloning, Molecular
Cytosol metabolism
DNA Primers genetics
Escherichia coli genetics
Escherichia coli metabolism
Escherichia coli Proteins
Fungal Proteins genetics
Gene Deletion
Gene Expression
Genes, Fungal
Green Fluorescent Proteins
HSP40 Heat-Shock Proteins
HSP70 Heat-Shock Proteins metabolism
Heat-Shock Proteins genetics
Luminescent Proteins genetics
Luminescent Proteins metabolism
Microscopy, Immunoelectron
Molecular Sequence Data
Phenotype
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae ultrastructure
Sequence Homology, Amino Acid
Fungal Proteins metabolism
Heat-Shock Proteins metabolism
Microbodies metabolism
Saccharomyces cerevisiae metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 142
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 9679141
- Full Text :
- https://doi.org/10.1083/jcb.142.2.421