Back to Search
Start Over
The HIS4 gene from the yeast Kluyveromyces lactis.
- Source :
-
Yeast (Chichester, England) [Yeast] 1998 May; Vol. 14 (7), pp. 687-91. - Publication Year :
- 1998
-
Abstract
- The Kluyveromyces lactis HIS4 gene was cloned by complementation of a Saccharomyces cerevisiae his4 mutant. Sequence analysis revealed a 2388 bp open reading frame encoding a single polypeptide predicted to encompass three distinct enzymatic activities (phosphoribosyl-AMP cyclohydrolase, phosphoribosyl-ATP pyrophosphohydrolase and histidinol dehydrogenase). This structural organization is strikingly similar to that of the His4 proteins from S. cerevisiae and Pichia pastoris. Transcript analysis detected a single mRNA species of 2.5 kb.
- Subjects :
- Alcohol Oxidoreductases
Amino Acid Sequence
Aminohydrolases
Cloning, Molecular
Fungal Proteins chemistry
Kluyveromyces chemistry
Molecular Sequence Data
Pyrophosphatases
RNA, Messenger metabolism
Saccharomyces cerevisiae genetics
Sequence Alignment
Sequence Analysis, DNA
Structure-Activity Relationship
Transcription Factors chemistry
Fungal Proteins genetics
Kluyveromyces genetics
Saccharomyces cerevisiae Proteins
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0749-503X
- Volume :
- 14
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Yeast (Chichester, England)
- Publication Type :
- Academic Journal
- Accession number :
- 9639316
- Full Text :
- https://doi.org/10.1002/(SICI)1097-0061(199805)14:7<687::AID-YEA261>3.0.CO;2-4