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An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast.
- Source :
-
Cell [Cell] 1998 Jun 12; Vol. 93 (6), pp. 1067-76. - Publication Year :
- 1998
-
Abstract
- Cohesion between sister chromatids during G2 and M phases depends on the "cohesin" protein Scc1p (Mcd1p). Loss of cohesion at the metaphase to anaphase transition is accompanied by Scc1p's dissociation from chromatids, which depends on proteolysis of Pds1p mediated by a ubiquitin protein ligase called the anaphase promoting complex (APC). We show that destruction of Pds1p is the APC's sole role in triggering Scc1p's dissociation from chromatids and that Pds1p forms a stable complex with a 180 kDa protein called Esp1p, which is essential for the dissociation of Scc1p from sister chromatids and for their separation. We propose that the APC promotes sister separation not by destroying cohesins but instead by liberating the "sister-separating" Esp1 protein from its inhibitor Pds1p.
- Subjects :
- Anaphase-Promoting Complex-Cyclosome
Cell Cycle Proteins metabolism
Cell Nucleus chemistry
Chromosomal Proteins, Non-Histone
Chromosomes, Fungal metabolism
Fungal Proteins chemistry
Fungal Proteins genetics
Ligases genetics
Ligases metabolism
Metaphase physiology
Molecular Weight
Mutation
Nuclear Proteins genetics
Phosphoproteins
Securin
Separase
Ubiquitin-Protein Ligases
Anaphase physiology
Chromatids metabolism
Endopeptidases
Fungal Proteins metabolism
Nuclear Proteins metabolism
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins
Ubiquitin-Protein Ligase Complexes
Subjects
Details
- Language :
- English
- ISSN :
- 0092-8674
- Volume :
- 93
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 9635435
- Full Text :
- https://doi.org/10.1016/s0092-8674(00)81211-8