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Growth hormone- and testosterone-dependent regulation of glutathione transferase subunit A5 in rat liver.
- Source :
-
The Biochemical journal [Biochem J] 1998 Jun 15; Vol. 332 ( Pt 3), pp. 763-8. - Publication Year :
- 1998
-
Abstract
- The class Alpha glutathione S-transferase (GST) subunit A5 is expressed in the livers of young male and female rats. After sexual maturation, this protein is no longer detectable in the livers of male rats, but is still expressed in female rats. We have previously demonstrated that the sexually dimorphic secretion of growth hormone regulates the levels of certain class Mu GSTs in rat liver, and this study was designed to investigate the hormonal regulation of GSTA5. Control and hypophysectomized rats of both sexes were used to study the role of growth hormone in the regulation of hepatic GSTA5; and the influence of testosterone on the expression of this same subunit was investigated in intact females and castrated males. Liver cytosols were subjected to SDS/PAGE and immunoblotting using antibodies directed towards rat (r)GSTA5, and to affinity purification on glutathione-Sepharose followed by reverse-phase HPLC in order to quantify the relative levels of rGSTA1, A2, A3, A4, M1 and M2 subunits. These analyses revealed that the expression of rGSTA5 is, indeed, regulated by both growth hormone and testosterone.
- Subjects :
- Animals
Blotting, Western
Castration
Chromatography, High Pressure Liquid
Female
Human Growth Hormone pharmacology
Humans
Hypophysectomy
Male
Rats
Rats, Sprague-Dawley
Testosterone pharmacology
Gene Expression Regulation, Enzymologic
Glutathione Transferase biosynthesis
Growth Hormone physiology
Liver enzymology
Testosterone physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-6021
- Volume :
- 332 ( Pt 3)
- Database :
- MEDLINE
- Journal :
- The Biochemical journal
- Publication Type :
- Academic Journal
- Accession number :
- 9620880
- Full Text :
- https://doi.org/10.1042/bj3320763