A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide.

Authors :: Desmarchelier PM
Bilge SS
Fegan N
Mills L
Vary JC Jr
Tarr PI
Source :: Journal of clinical microbiology [J Clin Microbiol] 1998 Jun; Vol. 36 (6), pp. 1801-4.
Publication Year :: 1998
Abstract: A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected < 1 CFU of E. coli O157 per ml in raw milk following enrichment.

Subjects :: Animals
Bacterial Typing Techniques
Enzyme-Linked Immunosorbent Assay
Escherichia coli O157 genetics
Humans
Immunoassay
Sensitivity and Specificity
Serotyping
Carbohydrate Epimerases genetics
Escherichia coli O157 isolation & purification
Milk microbiology
O Antigens biosynthesis
Polymerase Chain Reaction methods
Transaminases genetics

Full Text Access

Tools