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Evaluation of North American antibody detection tests for diagnosis of brucellosis in goats.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 1998 Jun; Vol. 36 (6), pp. 1716-22. - Publication Year :
- 1998
-
Abstract
- The sensitivities and specificities of 17 antibody detection tests for brucellosis in goats were estimated. Tests evaluated included the U.S. Department of Agriculture (USDA) card test with 8% cell concentration (8%Card), USDA rapid automated presumptive test (RAP), Mexican rose bengal plate tests with 8 and 3% cell concentrations (8%RB and 3%RB), French rose bengal plate test with 4.5% cell concentration (4.5%RB), USDA standard plate test (SPT), USDA buffered acidified plate agglutination test (BAPA), USDA and Mexican rivanol tests (URIV and MRIV), USDA standard tube tests with Brucella abortus and Brucella melitensis antigens (SATA and SATM), serum enzyme-linked immunosorbent assay (ELISA), USDA cold-fixation complement fixation tests with B. abortus and B. melitensis antigens (CFA and CFM), USDA and Mexican milk ring tests (UBRT and MBRT), and a milk ELISA. Test sensitivity was evaluated by using two groups of 10 goats experimentally infected with B. melitensis or B. abortus and monitored for 24 weeks. Specificity was evaluated by using 200 brucellosis-free nonvaccinated goats from 10 California herds. The 3%RB was considered a good screening test because of high sensitivity at week 24 postinfection (90%), ease of performance, and low cost. The cold-fixation CFA and CFM had 100% specificity in the field study and were considered appropriate confirmatory tests. The milk ELISA was significantly more sensitive (P < 0.05) than the UBRT and significantly more specific (P < 0.05) than the MBRT. The milk ELISA also had the advantage of objectivity and ease of interpretation.
- Subjects :
- Agglutination Tests veterinary
Animals
Antigens, Bacterial immunology
Brucellosis diagnosis
Brucellosis microbiology
Complement Fixation Tests veterinary
Enzyme-Linked Immunosorbent Assay veterinary
Evaluation Studies as Topic
Female
Goat Diseases microbiology
Goats
Male
Milk microbiology
Sensitivity and Specificity
United States
United States Department of Agriculture
Antibodies, Bacterial analysis
Brucella abortus immunology
Brucella abortus isolation & purification
Brucella melitensis immunology
Brucella melitensis isolation & purification
Brucellosis veterinary
Goat Diseases diagnosis
Serologic Tests veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 36
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 9620406
- Full Text :
- https://doi.org/10.1128/JCM.36.6.1716-1722.1998