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Murine Nkg2d and Cd94 are clustered within the natural killer complex and are expressed independently in natural killer cells.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1998 May 26; Vol. 95 (11), pp. 6320-5. - Publication Year :
- 1998
-
Abstract
- Natural killer (NK) cells express C-type lectin-like receptors, encoded in the NK gene complex, that interact with major histocompatibility complex class I and either inhibit or activate functional activity. Human NK cells express heterodimers consisting of CD94 and NKG2 family molecules, whereas murine NK cells express homodimers belonging to the Ly-49 family. The corresponding orthologues for other species, however, have not been described. In this report, we used probes derived from the expressed sequence tag database to clone C57BL/6-derived cDNAs homologous to human NKG2-D and CD94. Among normal tissues, murine NKG2-D and CD94 transcripts are highly expressed only in activated NK cells, including both Ly-49A+ and Ly-49A- subpopulations. Additionally, mNKG2-D is expressed in murine NK cell clones KY-1 and KY-2, whereas mCD94 expression is observed only in KY-1 cells but not KY-2. Last, we have finely mapped the physical location of the Cd94 (centromeric) and Nkg2d (telomeric) genes between Cd69 and the Ly49 cluster in the NK complex. Thus, these data indicate the expanding complexity of the NK complex and the corresponding repertoire of C-type lectin-like receptors on murine NK cells.
- Subjects :
- Amino Acid Sequence
Animals
Antigens, CD immunology
Humans
Membrane Glycoproteins immunology
Mice
Molecular Sequence Data
Multigene Family
NK Cell Lectin-Like Receptor Subfamily D
Receptors, Immunologic immunology
Sequence Alignment
Antigens, CD genetics
Gene Expression Regulation immunology
Killer Cells, Lymphokine-Activated immunology
Killer Cells, Natural immunology
Lectins, C-Type
Membrane Glycoproteins genetics
Receptors, Immunologic genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 95
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 9600963
- Full Text :
- https://doi.org/10.1073/pnas.95.11.6320