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Identification of Eucalyptus citriodora clones micropropagated in tissue culture.
- Source :
-
Nucleic acids symposium series [Nucleic Acids Symp Ser] 1997 (37), pp. 171-2. - Publication Year :
- 1997
-
Abstract
- The extent of genetic identity observed in the young individuals which were micropropagated from a single Eucalyptus individual was analyzed by using DNA-fingerprinting. Among 40,000 tissue-cultured-seedings of E.citriodora, 200 plants were randomly chosen so that each total DNA might be extracted from their leaves. Using these DNAs as template, PCR was performed with some primers we found in advance that leads polymorphism for DNA of E. citriodora. In this study, all over the 200 cases, the band pattern formed cDNA fragment on a gel after electrophoresis was the identical one mutually.
- Subjects :
- Base Sequence
Cell Culture Techniques methods
Clone Cells
DNA Fingerprinting
DNA Primers
DNA, Plant genetics
DNA, Plant isolation & purification
Electrophoresis, Agar Gel
Plant Leaves
Polymerase Chain Reaction methods
Random Amplified Polymorphic DNA Technique
Templates, Genetic
Trees cytology
DNA, Plant chemistry
Trees genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0261-3166
- Issue :
- 37
- Database :
- MEDLINE
- Journal :
- Nucleic acids symposium series
- Publication Type :
- Academic Journal
- Accession number :
- 9586054