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H2-M3 restricted presentation of a Listeria-derived leader peptide.
- Source :
-
The Journal of experimental medicine [J Exp Med] 1998 May 18; Vol. 187 (10), pp. 1711-9. - Publication Year :
- 1998
-
Abstract
- Protective immunity to infection by many intracellular pathogens requires recognition by cytotoxic T lymphocytes (CTLs) of antigens presented on major histocompatibility complex (MHC) class I molecules. To be presented for recognition by pathogen-specific CTLs, these antigens must gain access to the host cell class I processing pathway. In the case of intracellular bacterial pathogens, the majority of bacterial proteins are retained within the bacterial membrane and therefore remain inaccessible to the host cell for antigen processing. We have isolated a CTL clone from a C57BL/6 mouse infected with the intracellular gram-positive bacterium Listeria monocytogenes (LM) and have identified the source of the antigen. Using a genomic expression library, we determined that the clone recognizes an antigenic N-formyl peptide presented by the nonpolymorphic murine MHC class Ib molecule, H2-M3. Several lengths of this peptide were able to sensitize cells for lysis by this CTL clone. The source of this antigenic peptide is a 23-amino acid polypeptide encoded at the start of a polycistronic region. Analysis of mRNA secondary structure of this region suggests that this polypeptide may be a leader peptide encoded by a transcriptional attenuator.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred DBA
Molecular Sequence Data
Peptides immunology
T-Lymphocytes, Cytotoxic microbiology
Antigen Presentation
Antigens, Bacterial immunology
Histocompatibility Antigens Class I immunology
Listeria monocytogenes immunology
Listeriosis immunology
T-Lymphocytes, Cytotoxic immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1007
- Volume :
- 187
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The Journal of experimental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 9584149
- Full Text :
- https://doi.org/10.1084/jem.187.10.1711