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Interleukin-2-induces development of denditric cells from cord blood CD34+ cells.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 1998 May; Vol. 63 (5), pp. 620-30. - Publication Year :
- 1998
-
Abstract
- Dendritic cells (DC) have been shown to develop along a myeloid or lymphoid lineage of differentiation propagated from bone marrow or early thymic precursor cells with hematopoietic cytokines. In our study, we have induced growth and differentiation of DC from cord blood CD34+ cells initiated in interleukin-2 (IL-2) alone or in IL-2 + stem cell factor (SCF) + tumor necrosis factor alpha (TNF-alpha)-supplemented medium and cultured with IL-2 or IL-2 + SCF for 28-35 days. Dendritic morphology and antigenic phenotype of DC grown with IL-2 were characteristic for DC cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). Growth and differentiation of DC was followed by an increase in expression of MHC II and co-stimulating molecules CD80 and CD86. We have also shown the expression of the IL-2 receptor (IL-2R) gamma-chain in CD34+ cells after 2-3 days of culture with IL-2 alone. The co-expression of the IL-2R alpha, beta, and gamma subunits in both DC cultured with IL-2- or GM-CSF-containing cocktail of cytokines was also shown. The time curve for induction of IL-2R demonstrated low levels of subunit expression at the beginning of culture. The number of CD1a cells co-expressing CD25, CD122, and CDgamma increased to about 24-68 and to 78-95% after 21 and 28-35 days, respectively. Development of natural killer cells was shown along with DC. The proportion of CD56+ cells and cytotoxicity increased in a time-dependent manner.
- Subjects :
- Antigens, CD metabolism
Antigens, CD34 analysis
B7-1 Antigen metabolism
B7-2 Antigen
Cell Differentiation
Cell Division
Cells, Cultured
Dendritic Cells immunology
Humans
Immunity, Cellular
Immunophenotyping
Killer Cells, Natural cytology
Lymphocyte Culture Test, Mixed
Membrane Glycoproteins metabolism
Receptors, Interleukin-2 metabolism
Stem Cell Factor pharmacology
Tumor Necrosis Factor-alpha pharmacology
Dendritic Cells cytology
Fetal Blood cytology
Hematopoiesis drug effects
Interleukin-2 pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0741-5400
- Volume :
- 63
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 9581807
- Full Text :
- https://doi.org/10.1002/jlb.63.5.620