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Group A streptococcal Vir types are M-protein gene (emm) sequence type specific.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 1998 Apr; Vol. 36 (4), pp. 902-7. - Publication Year :
- 1998
-
Abstract
- The M-protein genes (emm genes) of 103 separate impetiginous Streptococcus pyogenes isolates were sequenced and the sequence types were compared to the types obtained by Vir typing. Vir typing is based on restriction fragment length polymorphism (RFLP) analysis of a 4- to 7-kb pathogenicity island encoding emm and other virulence genes. By using both HaeIII and HinfI to generate RFLP profiles, complete concordance between Vir type and emm sequence type was found. Comparison of the emm sequences with those in GenBank revealed new sequence types sharing less than 90% identity with known types. Diversity in the emm sequence was generated by corrected frameshift mutations, point mutations, and small in-frame mutations.
- Subjects :
- Amino Acid Sequence
Base Sequence
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Streptococcus pyogenes classification
Streptococcus pyogenes pathogenicity
Antigens, Bacterial
Bacterial Outer Membrane Proteins
Bacterial Proteins genetics
Carrier Proteins
Streptococcus pyogenes genetics
Virulence Factors
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 36
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 9542906
- Full Text :
- https://doi.org/10.1128/JCM.36.4.902-907.1998