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Absence of B7.1-CD28/CTLA-4-mediated co-stimulation in human NK cells.
- Source :
-
European journal of immunology [Eur J Immunol] 1998 Mar; Vol. 28 (3), pp. 780-6. - Publication Year :
- 1998
-
Abstract
- Recent studies have suggested that B7-CD28 interactions provide co-stimulatory signals for activation of NK cells. Transduction of the B7.1 (CD80) gene into tumor cells has been shown to trigger proliferation and cytotoxicity of murine NK cells and a human NK cell line, YT2C2. Therefore, transduction of the B7.1 gene into CD80-negative human squamous cell carcinomas of the head and neck (SCCHN) and its stable expression was expected to upregulate proliferation and cytotoxic activities of human NK cells. However, expression of the B7.1 receptors, CD28 and CTLA-4, could not be demonstrated on the surface or in the cytoplasm of normal human NK cells, irrespective of the state of their activation. In proliferation experiments or various cytotoxicity assays, utilizing highly purified human NK cells as responder or effector cells, no enhancement of NK cell generation or activity, respectively, by B7.1+ SCCHN was observed relative to non-transduced or LacZ gene-transduced SCCHN. In contrast, co-incubation of B7.1+ SCCHN targets with human NK cells induced significant inhibition of NK cell growth. Thus, the B7.1-CD28/CTLA-4 pathway is not involved in triggering of human adult NK cells.
- Subjects :
- Abatacept
Adult
Antigens, CD
CD28 Antigens physiology
CTLA-4 Antigen
Carcinoma, Squamous Cell immunology
Cytotoxicity, Immunologic
Head and Neck Neoplasms immunology
Humans
Immunity, Cellular
Immunophenotyping
Lymphocyte Activation
Tumor Cells, Cultured
Antigens, Differentiation physiology
B7-1 Antigen physiology
Immunoconjugates
Killer Cells, Natural immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2980
- Volume :
- 28
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 9541571
- Full Text :
- https://doi.org/10.1002/(SICI)1521-4141(199803)28:03<780::AID-IMMU780>3.0.CO;2-8