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Enzyme histochemical and immunohistochemical localization of carbonic anhydrase as a marker of ductal differentiation in the developing rat parotid gland.
- Source :
-
The Anatomical record [Anat Rec] 1998 Feb; Vol. 250 (2), pp. 190-8. - Publication Year :
- 1998
-
Abstract
- Background: Carbonic anhydrase has been localized to the acini and ducts of mature rat parotid glands. This enzyme has been associated with ion transport, a prominent function of striated and excretory ducts in salivary glands, suggesting that it might be used as a marker of ductal differentiation. The purpose of this study was histochemically to document developmental changes in carbonic anhydrase in the ducts of the rat parotid gland.<br />Methods: Parotid glands were excised from rats at representative developmental ages. Enzyme histochemistry was done on frozen sections fixed in acetone, and immunohistochemistry was performed with antibodies to human carbonic anhydrase isoenzymes I, II, and VI on paraffin sections of glands fixed in Helly's fluid.<br />Results: Carbonic anhydrase activity was weak until age 21 days after birth, when it had increased slightly in the acini and intercalated ducts and moderately in striated and excretory ducts. The adult pattern was attained by 28 days, in which reactions were moderate to strong in the striated and excretory ducts and modest in the acini and intercalated ducts. Immunohistochemical reactions were weak until 14 days, then increased rapidly, and by 28 days approached the adult pattern of virtually none in the acini and modest to moderately strong in the striated and excretory ducts. The order of reaction intensity of the antibodies was II > I > VI.<br />Conclusions: Carbonic anhydrase is a useful marker of the functional differentiation of the striated and excretory ducts of the developing rat parotid gland.
- Subjects :
- Animals
Biomarkers
Blotting, Western
Cell Differentiation
Cytoplasm enzymology
Cytoplasmic Granules enzymology
Female
Histocytochemistry
Immunoenzyme Techniques
Isoenzymes analysis
Male
Parotid Gland enzymology
Parotid Gland growth & development
Rats
Rats, Sprague-Dawley
Sex Characteristics
Carbonic Anhydrases analysis
Parotid Gland cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0003-276X
- Volume :
- 250
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Anatomical record
- Publication Type :
- Academic Journal
- Accession number :
- 9489780
- Full Text :
- https://doi.org/10.1002/(SICI)1097-0185(199802)250:2<190::AID-AR9>3.0.CO;2-7