Back to Search
Start Over
The messenger RNA encoding VGF, a neuronal peptide precursor, is rapidly regulated in the rat central nervous system by neuronal activity, seizure and lesion.
- Source :
-
Neuroscience [Neuroscience] 1998 Jan; Vol. 82 (1), pp. 7-19. - Publication Year :
- 1998
-
Abstract
- The VGF gene encodes a neuronal secretory-peptide precursor that is rapidly induced by neurotrophic growth factors and by depolarization in vitro. VGF expression in the animal peaks during critical periods in the developing peripheral and central nervous systems. To gain insight into the possible functions and regulation of VGF in vivo, we have used in situ hybridization to examine the regulation of VGF messenger RNA by experimental manipulations, and have found it to be regulated in the CNS by paradigms that affect electrical activity and by lesion. Inhibition of retinal electrical activity during the critical period of visual development rapidly repressed VGF messenger RNA in the dorsal lateral geniculate nucleus of the thalamus. In the adult, kainate-induced seizures transiently induced VGF messenger RNA in neurons of the dentate gyrus, hippocampus, and cerebral cortex within hours. Cortical lesion strongly induced VGF messenger RNA in ipsilateral cortex within hours, and strongly repressed expression in ipsilateral striatum. Ten days postlesion there was a delayed induction of VGF messenger RNA in a portion of deafferented striatum where compensatory cortical sprouting has been detected. Expression of the neuronal secretory-peptide precursor VGF is therefore modulated in vivo by monocular deprivation, seizure, and cortical lesion, paradigms which lead to neurotrophin induction, synaptic remodeling and axonal sprouting.
- Subjects :
- Animals
Central Nervous System metabolism
Excitatory Amino Acid Antagonists
Eye
Geniculate Bodies metabolism
Geniculate Bodies physiology
Image Processing, Computer-Assisted
In Situ Hybridization
Injections
Kainic Acid administration & dosage
Kainic Acid toxicity
Male
Neurons metabolism
Neuropeptides
RNA Probes
Rats
Rats, Inbred F344
Rats, Sprague-Dawley
Seizures chemically induced
Tetrodotoxin administration & dosage
Tetrodotoxin toxicity
Central Nervous System injuries
Central Nervous System physiology
Cerebral Cortex pathology
Neurons physiology
Protein Biosynthesis
Proteins
RNA, Messenger biosynthesis
Seizures pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0306-4522
- Volume :
- 82
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 9483499
- Full Text :
- https://doi.org/10.1016/s0306-4522(97)00280-7