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Level of viral antigen in blood leucocytes from cattle acutely infected with bovine viral diarrhoea virus.
- Source :
-
Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B [Zentralbl Veterinarmed B] 1997 Dec; Vol. 44 (10), pp. 583-90. - Publication Year :
- 1997
-
Abstract
- Blood samples from 24 calves undergoing experimental acute infection with a non-cytopathogenic bovine viral diarrhoea virus (BVDV) were examined for viral antigen in peripheral leucocytes with an enzyme-linked immunosorbent assay (ELISA), and for presence of virus in blood plasma in a cell culture assay. With the antigen ELISA, low positive values were detected in leucocytes sampled on days 3-4 from two of eight animals inoculated intranasally, and on days 11-13 from three of 16 animals inoculated intramuscularly. From 22 of the animals, low titres of BVDV were detected in blood plasma obtained 2-9 days after inoculation. All other samples, drawn between 2 and 21 days after inoculation, were negative for viral antigen. All animals seroconverted 3-4 weeks after inoculation, some after having shown mild and transient signs of disease.
- Subjects :
- Animals
Antibodies, Viral blood
Bovine Virus Diarrhea-Mucosal Disease diagnosis
Bovine Virus Diarrhea-Mucosal Disease virology
Cattle
Diarrhea Viruses, Bovine Viral isolation & purification
Enzyme-Linked Immunosorbent Assay veterinary
Female
Antigens, Viral blood
Bovine Virus Diarrhea-Mucosal Disease immunology
Diarrhea Viruses, Bovine Viral immunology
Leukocytes virology
Subjects
Details
- Language :
- English
- ISSN :
- 0514-7166
- Volume :
- 44
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B
- Publication Type :
- Academic Journal
- Accession number :
- 9467300
- Full Text :
- https://doi.org/10.1111/j.1439-0450.1997.tb01011.x