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Cellular localization and expression of the serotonin transporter in mouse brain.
- Source :
-
Brain research [Brain Res] 1997 Dec 19; Vol. 778 (2), pp. 338-45. - Publication Year :
- 1997
-
Abstract
- The high-affinity serotonin (5-HT) transporter (5-HTT) plays an important role in the removal of extracellular serotonin, thereby modulating and terminating the action of this neurotransmitter at various pre- and post-synaptic serotonergic receptors and heteroreceptors. In order to characterize the anatomical distribution of the 5-HTT in mouse brain, in situ hybridization histochemistry using 35S-labeled riboprobes was performed. These results were compared with 5-HTT binding site distribution as evaluated by [125I]RTI-55 autoradiography. High levels of 5-HTT mRNA were detected in all brain stem raphe nuclei, with variations in labeling among the various subnuclei. Those brain areas known to possess serotonergic cell bodies stained intensely for both 5-HTT mRNA and 5-HTT binding sites. In contrast to previous findings in rat brain, the highest densities of 5-HTT sites were found in areas outside the raphe complex, particularly in the substantia nigra, globus pallidus, and superior colliculi.
- Subjects :
- Animals
Carrier Proteins analysis
Cerebellum chemistry
Cloning, Molecular
Gene Expression
Globus Pallidus chemistry
In Situ Hybridization
Iodine Radioisotopes
Male
Membrane Glycoproteins analysis
Mice
Nerve Tissue Proteins analysis
Nerve Tissue Proteins genetics
RNA Probes
RNA, Messenger analysis
Raphe Nuclei chemistry
Serotonin Plasma Membrane Transport Proteins
Substantia Nigra chemistry
Superior Colliculi chemistry
Brain Chemistry physiology
Carrier Proteins genetics
Membrane Glycoproteins genetics
Membrane Transport Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0006-8993
- Volume :
- 778
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 9459551
- Full Text :
- https://doi.org/10.1016/s0006-8993(97)01080-9