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Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA.
- Source :
-
The EMBO journal [EMBO J] 1998 Feb 02; Vol. 17 (3), pp. 797-807. - Publication Year :
- 1998
-
Abstract
- Site-specific 2'-O-ribose methylation of eukaryotic rRNAs is guided by small nucleolar RNAs (snoRNAs). The methylation guide snoRNAs carry long perfect complementaries to rRNAs. These antisense elements are located either in the 5' half or in the 3' end region of the snoRNA, and are followed by the conserved D' or D box motifs, respectively. An uninterrupted helix formed between the rRNA and the antisense element of the snoRNA, in conjunction with the adjacent D' or D box, constitute the recognition signal for the putative methyltransferase. Here, we have identified an additional essential box element common to methylation guide snoRNAs, termed the C' box. We show that the C' box functions in concert with the D' box and plays a crucial role in the methyltransfer reaction directed by the upstream antisense element and the D' box. We also show that an internal fragment of U24 methylation guide snoRNA, encompassing the upstream antisense element and the D' and C' box motifs, can support the site-specific methylation of rRNA. This strongly suggests that the C box of methylation guide snoRNAs plays an essential role in the methyltransfer reaction guided by the 3'-terminal antisense element and the D box of the snoRNA.
- Subjects :
- Base Sequence
Binding Sites genetics
Binding Sites physiology
Cell Nucleolus chemistry
Cell Nucleolus metabolism
Humans
Methylation
Molecular Sequence Data
Nucleic Acid Conformation
RNA Precursors genetics
RNA Precursors metabolism
RNA, Antisense genetics
RNA, Ribosomal, 28S metabolism
RNA, Small Nuclear analysis
Saccharomyces cerevisiae chemistry
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae physiology
Sequence Homology, Nucleic Acid
RNA, Small Untranslated
RNA, Ribosomal, 28S genetics
RNA, Small Nuclear genetics
Ribose metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0261-4189
- Volume :
- 17
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The EMBO journal
- Publication Type :
- Academic Journal
- Accession number :
- 9451004
- Full Text :
- https://doi.org/10.1093/emboj/17.3.797