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Heterogeneity of the inhibitory effects of IL-4 in two novel B lineage acute lymphoblastic leukemia cell lines.
- Source :
-
Leukemia research [Leuk Res] 1997 Nov-Dec; Vol. 21 (11-12), pp. 1037-46. - Publication Year :
- 1997
-
Abstract
- The present study describes two novel cell lines, DUNATIS and SILVANUS, established from B lineage acute lymphoblastic leukemia patients. Respectively, DUNATIS and SILVANUS display an early pre-B cell and a pre-B cell phenotype. Spontaneous DNA replication of both cell lines was strongly inhibited by IL-4. This effect was directly mediated by IL-4 and exerted through the CD124 IL-4 receptor chain. Notably, IL-4 was associated with rapid cell death and reduction of cellularity in DUNATIS, whereas these parameters were considerably less pronounced and only observed after longer-term exposure of the SILVANUS cells to IL-4. In addition to these differences, although both cell lines expressed FES oncoprotein, a 100 kDa protein associated with FES was strikingly found to be tyrosine-phosphorylated in response to IL-4 exclusively in DUNATIS cells. These data demonstrate that IL-4 displays heterogenous effects on leukemic B cell precursors responsive to inhibition of DNA synthesis via IL-4 mediated engagement of the CD124 receptor chain. The present findings may be of use for appreciation of the effects of IL-4 in B lineage ALL, and the novel cell lines could represent a model for further identification of target molecules in IL-4 signalling.
- Subjects :
- Adult
Aged
Burkitt Lymphoma metabolism
Cell Division drug effects
Cell Survival drug effects
DNA Replication drug effects
DNA, Neoplasm biosynthesis
DNA, Neoplasm drug effects
Female
Humans
Male
Neoplasm Proteins metabolism
Phenotype
Phosphorylation drug effects
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma metabolism
Proto-Oncogene Proteins metabolism
Proto-Oncogene Proteins c-fes
Tumor Cells, Cultured drug effects
Burkitt Lymphoma drug therapy
Burkitt Lymphoma pathology
Interleukin-4 pharmacology
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma drug therapy
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma pathology
Protein-Tyrosine Kinases
Subjects
Details
- Language :
- English
- ISSN :
- 0145-2126
- Volume :
- 21
- Issue :
- 11-12
- Database :
- MEDLINE
- Journal :
- Leukemia research
- Publication Type :
- Academic Journal
- Accession number :
- 9444937
- Full Text :
- https://doi.org/10.1016/s0145-2126(97)00099-4