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Nuclear factor (NF)-kappa B2 (p100/p52) is required for normal splenic microarchitecture and B cell-mediated immune responses.
- Source :
-
The Journal of experimental medicine [J Exp Med] 1998 Jan 19; Vol. 187 (2), pp. 185-96. - Publication Year :
- 1998
-
Abstract
- The nfkb2 gene is a member of the Rel/NF-kappa B family of transcription factors. COOH-terminal deletions and rearrangements of this gene have been associated with the development of human cutaneous T cell lymphomas, chronic lymphocytic leukemias, and multiple myelomas. To further investigate the function of NF-kappa B2, we have generated mutant mice carrying a germline mutation of the nfkb2 gene by homologous recombination. NF-kappa B2-deficient mice showed a marked reduction in the B cell compartment in spleen, bone marrow, and lymph nodes. Moreover, spleen and lymph nodes of mutant mice presented an altered architecture, characterized by diffuse, irregular B cell areas and the absence of discrete perifollicular marginal and mantle zones; the formation of secondary germinal centers in spleen was also impaired. Proliferation of NF-kappa B2-deficient B cells was moderately reduced in response to lipopolysaccharide, anti-IgD-dextran, and CD40, but maturation and immunoglobulin switching were normal. However, nfkb2 (-/-) animals presented a deficient immunological response to T cell-dependent and -independent antigens. These findings indicate an important role of NF-kappa B2 in the maintenance of the peripheral B cell population, humoral responses, and normal spleen architecture.
- Subjects :
- Animals
B-Lymphocyte Subsets metabolism
Bone Marrow Cells immunology
Bone Marrow Cells pathology
CD40 Antigens physiology
Epitopes genetics
Female
Germinal Center pathology
Immunity, Cellular genetics
Immunoglobulins biosynthesis
Lipopolysaccharides pharmacology
Lymph Nodes immunology
Lymph Nodes pathology
Lymphocyte Activation genetics
Lymphopenia genetics
Lymphopenia immunology
Lymphopenia pathology
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, Knockout
Mutagenesis, Insertional immunology
NF-kappa B immunology
NF-kappa B p52 Subunit
Receptors, Antigen, B-Cell pharmacology
Signal Transduction immunology
B-Lymphocyte Subsets immunology
B-Lymphocyte Subsets pathology
NF-kappa B deficiency
NF-kappa B genetics
Spleen immunology
Spleen pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1007
- Volume :
- 187
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of experimental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 9432976
- Full Text :
- https://doi.org/10.1084/jem.187.2.185