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NotI linking/jumping clones of human chromosome 3: mapping of the TFRC, RAB7 and HAUSP genes to regions rearranged in leukemia and deleted in solid tumors.
- Source :
-
FEBS letters [FEBS Lett] 1997 Dec 15; Vol. 419 (2-3), pp. 181-5. - Publication Year :
- 1997
-
Abstract
- By applying the 'recognition mask' strategy to 300 mammalian sequences containing NotI sites we demonstrated that 5' ends of genes are highly enriched in NotI sites. A NotI linking clone NL2-252 (D3S1678) containing transferrin receptor (TFRC) gene was used as an initial point for chromosomal jumping. One of the jumping clones, J21-045 traverses 210 kbp and links NL2-252 to NL26 (D3S1632), a NotI linking clone containing highly polymorphic sequences. The TFRC gene was mapped to 3q29, close to the telomeric marker D3S2344, by linkage analysis, a panel of hybrid cell lines, GeneBridge 4 panel and FISH. Clone NLM-007 (D3S4302) was found to contain ras-homologous gene RAB7. By FISH and a panel of hybrid cell lines this gene was mapped to 3q21. This region is of particular interest due to frequent rearrangements in different types of leukemia. Clone L2-081 (D3S4283) containing new member of ubiquitin-specific proteases (HAUSP gene) was localized in 3p21 inspiring further investigation of involvement of this gene in development of lung and renal carcinomas.
- Subjects :
- Cloning, Molecular
Gene Rearrangement
Humans
Molecular Sequence Data
Ubiquitin Thiolesterase
Ubiquitin-Specific Peptidase 7
rab7 GTP-Binding Proteins
Chromosome Mapping
Chromosomes, Human, Pair 3
Endopeptidases genetics
GTP-Binding Proteins genetics
Gene Library
Genome, Human
Leukemia genetics
Neoplasms genetics
Receptors, Transferrin genetics
rab GTP-Binding Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 419
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 9428630
- Full Text :
- https://doi.org/10.1016/s0014-5793(97)01449-x