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CIF150, a human cofactor for transcription factor IID-dependent initiator function.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 1998 Jan; Vol. 18 (1), pp. 233-9. - Publication Year :
- 1998
-
Abstract
- The transcription factor IID (TFIID) complex is highly conserved between the Drosophila and mammalian systems. A mammalian homolog has been described for all the Drosophila TATA box-binding protein-associated factors (TAFs), with the exception of dTAF(II)150. We previously reported the identification of CIF, an essential cofactor for TFIID-dependent transcription from promoters containing initiator (Inr) elements. Here we describe the molecular cloning of CIF150, the human homolog of dTAF(II)150, and present biochemical evidence that this factor is involved in Inr activity. CIF150 is capable of mediating TFIID-dependent Inr activity in a complementation assay, and a protein fraction lacking Inr activity lacks detectable amounts of CIF150. Despite the striking similarity to dTAF(II)150, CIF150 does not appear to be associated with human TFIID. However, in vitro binding assays revealed a specific and direct interaction between CIF150 and hTAF(II)135. This interaction might be structurally important for the functional interaction between CIF150 and human TFIID, since CIF150 stabilizes TFIID binding to a core promoter.
- Subjects :
- Amino Acid Sequence
HeLa Cells
Humans
Molecular Sequence Data
Sequence Alignment
TATA Box
Transcription Factor TFIID
DNA-Binding Proteins genetics
Promoter Regions, Genetic
TATA-Binding Protein Associated Factors
Transcription Factors genetics
Transcription Factors, TFII genetics
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 0270-7306
- Volume :
- 18
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 9418870
- Full Text :
- https://doi.org/10.1128/MCB.18.1.233