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Alpha-melanocyte-stimulating hormone and endothelin-1 have opposing effects on melanocyte adhesion, migration, and pp125FAK phosphorylation.
- Source :
-
Experimental cell research [Exp Cell Res] 1997 Nov 25; Vol. 237 (1), pp. 19-28. - Publication Year :
- 1997
-
Abstract
- Recent reports show that alpha-MSH (melanocyte-stimulating hormone) is mitogenic and melanogenic for normal human melanocytes, and that this effect is mediated through binding to the melanocortin receptor (MC1R) and activation of cAMP formation. alpha-MSH has also been shown to induce changes in cell shape in melanocytes and melanoma cells, particularly increased dendricity, suggesting a potential role for alpha-MSH in melanocyte-matrix interactions and pigment transfer through reorganization of the melanocyte actin filament cytoskeleton. In this report we show that the potent alpha-MSH analog (Nle4, D-Phe7)-alpha-MSH (NDP-MSH) induces reorganization of the actin stress fiber cytoskeleton in treated human melanocytes and that this reorganization is associated with increased adhesion to fibronectin (FN). Because most melanocyte growth factors act synergistically on melanocyte mitogenesis, we also sought to determine the effect of the melanocyte mitogen endothelin-1 (ET-1) on the melanocyte actin cytoskeleton, melanocyte adhesion, and melanocyte migration. We show that ET-1, which increases melanocyte migration on FN, has opposite effects on melanocyte adhesion to FN compared with NDP-MSH and that endothelin-1-induced actin reorganization is distinct from that observed following NDP-MSH treatment. Finally, we show that focal adhesion kinase (pp125FAK), a nonreceptor tyrosine kinase associated with focal contact formation and cell migration, is phosphorylated on tyrosine residues after treatment of melanocytes with ET-1, but not NDP-MSH. These data indicate that while alpha-MSH and ET-1 act synergistically to modulate melanocyte proliferation, they have opposite effects on melanocyte-matrix interactions.
- Subjects :
- Actins drug effects
Actins metabolism
Bucladesine pharmacology
Cell Adhesion drug effects
Cell Movement drug effects
Cells, Cultured
Cytoskeleton physiology
Extracellular Matrix physiology
Fibronectins
Focal Adhesion Kinase 1
Focal Adhesion Protein-Tyrosine Kinases
Humans
Infant, Newborn
Male
Melanocytes cytology
Melanocytes drug effects
Phosphorylation
Receptors, Corticotropin physiology
Receptors, Melanocortin
Skin cytology
Cell Adhesion Molecules metabolism
Endothelin-1 pharmacology
Melanocytes physiology
Protein-Tyrosine Kinases metabolism
alpha-MSH analogs & derivatives
alpha-MSH pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-4827
- Volume :
- 237
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Experimental cell research
- Publication Type :
- Academic Journal
- Accession number :
- 9417862
- Full Text :
- https://doi.org/10.1006/excr.1997.3765