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Synthesis and enzymatic stability of phosphodiester-linked peptide-oligonucleotide hybrids.

Authors :
Robles J
Maseda M
Beltrán M
Concernau M
Pedroso E
Grandas A
Source :
Bioconjugate chemistry [Bioconjug Chem] 1997 Nov-Dec; Vol. 8 (6), pp. 785-8.
Publication Year :
1997

Abstract

Nucleopeptides Ac-Tyr(p3' dACGT)-Ala-Phe-Gly-NH2, Ac-Thr(p3'dACGT)-Ala-Phe-Gly-OH, Ac-Ser(p3'dACGT)-Ala-Phe-Gly-OH, and Phac-Hse(p3'dACGT)-Ala-Phe-Gly-OH, in which the 3'-end of a tetradeoxyribonucleotide is linked by a phosphodiester bond to a hydroxylated amino acid, were synthesized using a stepwise solid-phase methodology to study the influence of the linking amino acid on their stability to 3'-exonucleases. HPLC analysis of the reaction crudes after treatment of each nucleopeptide with snake venom phosphodiesterase showed that the lability of the amino acid-nucleoside linkage increases in the order Thr < Ser < Hse < Tyr.

Details

Language :
English
ISSN :
1043-1802
Volume :
8
Issue :
6
Database :
MEDLINE
Journal :
Bioconjugate chemistry
Publication Type :
Academic Journal
Accession number :
9404649
Full Text :
https://doi.org/10.1021/bc970051u