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beta-dystrobrevin, a new member of the dystrophin family. Identification, cloning, and protein associations.
beta-dystrobrevin, a new member of the dystrophin family. Identification, cloning, and protein associations.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1997 Dec 12; Vol. 272 (50), pp. 31561-9. - Publication Year :
- 1997
-
Abstract
- Dystrophin, the protein disrupted in Duchenne muscular dystrophy, is one of several related proteins that are key components of the submembrane cytoskeleton. Three dystrophin-related proteins (utrophin, dystrophin-related protein-2 (DRP2), and dystrobrevin) have been described. Here, we identify a human gene on chromosome 2p22-23 that encodes a novel protein, beta-dystrobrevin, with significant homology to the other known dystrobrevin (now termed alpha-dystrobrevin). Sequence alignments including this second dystrobrevin strongly support the concept that two distinct subfamilies exist within the dystrophin family, one composed of dystrophin, utrophin, and DRP2 and the other composed of alpha- and beta-dystrobrevin. The possibility that members of each subfamily form distinct protein complexes was examined by immunopurifying dystrobrevins and dystrophin. A beta-dystrobrevin antibody recognized a protein of the predicted size (71 kDa) that copurified with the dystrophin short form, Dp71. Thus, like alpha-dystrobrevin, beta-dystrobrevin is likely to associate directly with dystrophin. alpha- and beta-dystrobrevins failed to copurify with each other, however. These results suggest that members of the dystrobrevin subfamily form heterotypic associations with dystrophin and raise the possibility that pairing of a particular dystrobrevin with dystrophin may be regulated, thereby providing a mechanism for assembly of distinct submembrane protein complexes.
- Subjects :
- Amino Acid Sequence
Base Sequence
Brain Chemistry
Chromosome Mapping
Cytoskeletal Proteins chemistry
Dystrophin chemistry
Humans
Membrane Proteins chemistry
Membrane Proteins classification
Models, Molecular
Molecular Sequence Data
Sequence Alignment
Utrophin
Cloning, Molecular
Dystrophin-Associated Proteins
Membrane Proteins genetics
Muscle Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 272
- Issue :
- 50
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 9395493
- Full Text :
- https://doi.org/10.1074/jbc.272.50.31561