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The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases.
- Source :
-
Cell [Cell] 1997 Nov 14; Vol. 91 (4), pp. 457-66. - Publication Year :
- 1997
-
Abstract
- We identified a complex in S. cerevisiae, the "exosome," consisting of the five essential proteins Rrp4p, Rrp41p, Rrp42p, Rrp43p, and Rrp44p (Dis3p). Remarkably, four of these proteins are homologous to characterized bacterial 3'-->5' exoribonucleases; Rrp44p is homologous to RNase II, while Rrp41p, Rrp42p, and Rrp43p are related to RNase PH. Recombinant Rrp4p, Rrp44p, and Rrp41p are 3'-->5' exoribonucleases in vitro that have distributive, processive, and phosphorolytic activities, respectively. All components of the exosome are required for 3' processing of the 5.8S rRNA. Human Rrp4p is found in a comparably sized complex, and expression of the hRRP4 gene in yeast complements the rrp4-1 mutation. We conclude that the exosome constitutes a highly conserved eukaryotic RNA processing complex.
- Subjects :
- Amino Acid Sequence
Exoribonucleases chemistry
Exoribonucleases genetics
Exoribonucleases isolation & purification
Fungal Proteins chemistry
Fungal Proteins genetics
Fungal Proteins isolation & purification
Genetic Complementation Test
HeLa Cells
Humans
Molecular Sequence Data
Molecular Weight
Mutation
RNA, Ribosomal, 5.8S metabolism
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins isolation & purification
Recombinant Fusion Proteins metabolism
Exoribonucleases metabolism
Fungal Proteins metabolism
Multienzyme Complexes metabolism
RNA Processing, Post-Transcriptional physiology
Saccharomyces cerevisiae enzymology
Saccharomyces cerevisiae Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0092-8674
- Volume :
- 91
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 9390555
- Full Text :
- https://doi.org/10.1016/s0092-8674(00)80432-8