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The normally silent sigma54 promoters upstream of the pilE genes of both Neisseria gonorrhoeae and Neisseria meningitidis are functional when transferred to Pseudomonas aeruginosa.
- Source :
-
Gene [Gene] 1997 Oct 01; Vol. 198 (1-2), pp. 89-97. - Publication Year :
- 1997
-
Abstract
- The pilE gene encodes the pilin subunit in Neisseria gonorrhoeae and Neisseria meningitidis. Transcriptional analysis of promoters upstream of pilE in N. gonorrhoeae has been described previously (Fyfe et al. (1995) J. Bacteriol. 177, 3781-3787). Transcription from the sigma54-dependent promoter P3 was detected in Pseudomonas aeruginosa. Here we show that this transcription is dependent on the P. aeruginosa transcriptional activator PilR, and a specific upstream sequence with a high degree of similarity to the PilR-binding site found upstream of the P. aeruginosa pilin gene. This implies there is an upstream activator site (UAS) present 5' of pilE. Sequencing upstream of the N. meningitidis MC58 c2 pilE gene shows this region to be very similar to that in N. gonorrhoeae. P3 and the UAS are conserved, although insertions were noted on either side of the UAS. Transcriptional analysis has shown that the N. meningitidis P3 promoter is used in P. aeruginosa, provided PilR and an upstream region that includes sequence similar to the UAS are present. Transcription from the N. meningitidis PpilE is stronger than from the N. gonorrhoeae equivalent. N. meningitidis uses the sigma70 promoter P1 to transcribe pilE.
- Subjects :
- Bacterial Adhesion
Base Sequence
DNA, Bacterial genetics
Fimbriae Proteins
Molecular Sequence Data
Transcription Factors genetics
Transcription, Genetic
Bacterial Outer Membrane Proteins genetics
Bacterial Proteins genetics
Gene Expression Regulation, Bacterial
Membrane Glycoproteins genetics
Neisseria gonorrhoeae genetics
Neisseria meningitidis genetics
Promoter Regions, Genetic
Pseudomonas aeruginosa genetics
Sigma Factor genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1119
- Volume :
- 198
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 9370268
- Full Text :
- https://doi.org/10.1016/s0378-1119(97)00297-7