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Alternative splicing of Pax6 in bovine eye and evolutionary conservation of intron sequences.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1997 Nov 07; Vol. 240 (1), pp. 196-202. - Publication Year :
- 1997
-
Abstract
- Pax-6 is essential for eye development and for tissue-specific gene expression within the eye. Splicing of an alternative exon (5a) leads to variant DNA binding specificity in Pax6. Here we show that in the mature bovine eye the relative abundance of the alternative Pax6-5a mRNA varies markedly, particularly between the lens and iris. Additional alternative splicing products were identified which may lead to "domain swapping" or truncation of Pax6 proteins. Comparison of amphibian (Xenopus) and mammalian (bovine) Pax-6 genes revealed highly conserved intronic sequences flanking exon 5a, including potential lariat sites which may have a role in the mechanism of alternative splicing.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cattle
DNA-Binding Proteins chemistry
DNA-Binding Proteins isolation & purification
Molecular Sequence Data
PAX6 Transcription Factor
Paired Box Transcription Factors
Phylogeny
RNA, Messenger genetics
Repressor Proteins
Xenopus laevis genetics
Alternative Splicing
Conserved Sequence
DNA-Binding Proteins genetics
Evolution, Molecular
Eye Proteins genetics
Homeodomain Proteins
Introns
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 240
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 9367909
- Full Text :
- https://doi.org/10.1006/bbrc.1997.7623