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Expression of the coat protein of potato virus X from a dicistronic mRNA in transgenic potato plants.
- Source :
-
The Journal of general virology [J Gen Virol] 1997 Nov; Vol. 78 ( Pt 11), pp. 3051-9. - Publication Year :
- 1997
-
Abstract
- Transgenic potato plants were generated which express a dicistronic mRNA corresponding to the 8 kDa protein and coat protein (CP) genes of potato virus X (PVX). Northern blot analysis of RNA isolated from these plants indicated that both the 8 kDa protein and the CP are translated from this dicistronic mRNA. Expression of both of these proteins in transgenic plants was demonstrated by Western blot analysis, and suggests that translation of CP takes place either by initiation by internal entry of ribosomes or by a termination/reinitiation mechanism. CP was detected in protoplasts electroporated with RNA transcripts of the dicistronic construct in the presence or absence of a stable hairpin structure at the 5' terminus, suggesting that the former model is more likely. Similar results were obtained when a DNA fragment containing the PVX CP leader sequence was placed between two reporter genes in the transient assay system. These results suggest that potexvirus CP expression may take place from the genomic RNA as well as from the subgenomic RNA.
- Subjects :
- Base Sequence
Capsid biosynthesis
Gene Expression Regulation, Plant
Gene Expression Regulation, Viral
Molecular Sequence Data
Plants, Genetically Modified
Potexvirus metabolism
RNA, Messenger genetics
Solanum tuberosum genetics
Solanum tuberosum metabolism
Capsid genetics
Potexvirus genetics
RNA, Viral genetics
Solanum tuberosum virology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 78 ( Pt 11)
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 9367393
- Full Text :
- https://doi.org/10.1099/0022-1317-78-11-3051